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Next Generation Sequencing Techniques allow to analyse the transcribed parts of the DNA. This requires to reconstruct the transcripts from the short reads that are obtained by the sequencing technique. The estimation of the quantities of the transcripts allows the comparison of transcript abundance of different samples.
The course introduces the theory and practice of Transcriptomics including best practices and examples from UFZ.
The wat lab procedures for obtaining transcriptome data from samples will be explained, e.g. sample preparation and sequencing with an Illumina sequencer.
The theory of the bioinformatics analysis of sequencing data will be explained. Practical analyses of transcriptimics data will be performed using the UFZ Galaxy server. The bioinformatics part will cover the following topics:
Theoretical input, group exercises
Participation fees do not apply to cooperation partners, i.e. PhD candidates of SENSE partner WIMEK.
The current conditions of participation and use apply.
Please note: In order to register, a personal account has to be created first.