Research School for Socio-Economic and
Natural Sciences of the Environment
Research School for Socio-Economic and
Natural Sciences of the Environment
Agenda

Introduction to Transcriptomics

Date: 02 September 2019 - 03 September 2019
Location: UFZ Leipzig, Germany

Next Generation Sequencing Techniques allow to analyse the transcribed parts of the DNA. This requires to reconstruct the transcripts from the short reads that are obtained by the sequencing technique. The estimation of the quantities of the transcripts allows the comparison of transcript abundance of different samples.

Contents

The course introduces the theory and practice of Transcriptomics including best practices and examples from UFZ.

The wat lab procedures for obtaining transcriptome data from samples will be explained, e.g. sample preparation and sequencing with an Illumina sequencer.

The theory of the bioinformatics analysis of sequencing data will be explained. Practical analyses of transcriptimics data will be performed using the UFZ Galaxy server. The bioinformatics part will cover the following topics:

  • Quality control
  • Analysis of RNA sequencing data using a reference genome
  • Reconstruction of transcripts without reference transcriptome (de novo)
  • Analysis of differentially expressed genes

Didactic Aims

  • Understand sample preparation and next generation sequencing of transcriptomics data
  • Using the UFZ Galaxy Server
  • Analyse transcriptomics data using the Galaxy server
  • Understand the used bioinformatics methods

Didactic Elements

Theoretical input, group exercises

Target Group

  • Doctoral Researchers
  • Postdocs & Senior Scientists
  • Technical Staff

Complementary Courses

  • Introduction to Amplicon and Metagenome Analyses

> More information about this course

> More UFZ courses

Registration and fee

Participation fees do not apply to cooperation partners, i.e. PhD candidates of SENSE partner WIMEK.
The current conditions of participation and use apply.
Please note: In order to register, a personal account has to be created first.





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