Title: Long-lived luminescence: a tool to improve concentration detection limits in capillary electrophoresis

Primary Researcher: Koos Kuijt
Email address: kuijt@chem.vu.nl
Submitted on: October 3, 2006

Start date: 01 November 1997
End date: 01 November 2001

Introduction:

Due to capillary dimensions, the sensitivity of optical detection methods - like absorption detection - in capillary electrophoresis (CE) is limited. To obtain the low detection limits often required in environmental analysis, alternative detection strategies have to be involved.

Aim:

The project is aimed at the implementation of sensitized and quenched phosphorescence detection in capillary electrophoresis in order to lower the detection limits achievable in CE.

Research:

Adapted setups required to enable phosphorescence detection in CE were developed. Sensitized and quenched phosphorescence were performed by using phosphorescing compounds in the CE-buffer to provide a continuous phosphorescence background. Relevant test analytes included naphtha-lenesulfonates, nitrophenols and (di)thiocarbamates; these compounds could be detected with detection limits in the low ppb-range (1–10 ppb).

Future Research

Currently, the potential of 266-nm laser-based phosphorescence detection is investigated. This should allow for even lower detection limits.

Funding via University

Yes

External Funding Sponsors

NWO