Research School for Socio-Economic and
Natural Sciences of the Environment
Research School for Socio-Economic and
Natural Sciences of the Environment
Agenda

Advanced Proteomics

Date: 07 May 2019 - 10 May 2019
Location: Wageningen University

Proteomics is the study of the proteome, the set of proteins expressed in an organism. The proteome can change dramatically depending on biological conditions. Proteomics is able to capture detailed snapshots of the proteome using mass spectrometry. The rapid development in technical advances of instrumentation has led to new bioinformatics approaches. In this course the focus will be on sample preparation and data analysis.

Course format

The course is a 4 days hands-on course with a maximum of 16 participants (PhD’s and postdocs are the focus group). In the morning sessions lectures on mass spectrometry and proteomics applications and in the afternoon sessions practical work are scheduled. Each participant can bring their own sample as one slice or spot of a SDS protein 1 or 2D gel. Samples will be prepared by the participants and measured using splitless nano-LC Orbitrap FTMSMS. 

Target group

PhD candidates or Postdocs interested in this course should work on proteomics projects or plan to use proteomics in the near future and have a strong background in nutrition, biochemistry, physiology, molecular biology or a comparable discipline.

Aim of the course

Participants will learn

1.how to make state of the art proteomics samples
2.perform proteomic data analysis using MaxQuant
3.integrate results into biological research

Programme topics

  • Sample preparation protocols
  • Proteomics instrumentation
  • Quantitative techniques
  • MaxQuant and Perseus
  • Integration of ~omics data
  • New mass spectrometric tools in proteomics

Intended credits1.4 ECTS
Course organisationThe Graduate School VLAG and the Laboratory of Biochemistry, Wageningen University and Research, Wageningen
More informationCourse website